| COX-2抑制剂对PC-3细胞的放射增敏作用研究 |
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| 引用本文： | 宋轶鹏,臧茹琨,马金波.COX-2抑制剂对PC-3细胞的放射增敏作用研究[J].西南科技大学学报(哲学社会科学版),2011,9(6). |
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| 作者姓名： | 宋轶鹏 臧茹琨 马金波 |
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| 作者单位： | 青岛大学附属烟台毓璜顶医院放疗科; |
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| 基金项目： | 山东省科技发展项目编号2004GG3202019 |
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| 摘 要： | 目的应用选择性环氧合酶-2(COX-2)抑制剂NS-398作用于胰腺癌细胞株PC-3,观察其放射增敏作用,并对其可能增敏机制进行初步探讨。方法体外培养胰腺癌细胞株PC-3;以流式细胞术(FCM)单抗标记技术检测PC-3细胞的COX-2表达水平;以MTT实验检测细胞增殖抑制情况及NS-398对细胞放射敏感性的影响;以FCM分析细胞周期变化,以RT-PCR分析细胞周期相关蛋白P21表达变化。结果 FCM显示PC-3细胞存在COX-2的异常高表达;MTT实验显示NS-398可显著增强PC-3细胞的放射敏感性;FCM显示NS-398联合放射组G0-G1期比率上调;RT-PCR显示NS-398联合放射组细胞内P21mRNA表达上调。结论选择性COX-2抑制剂NS-398可增强胰腺癌细胞PC-3的放射敏感性,其机制可能与上调P21mRNA表达,进而诱导细胞周期阻滞于G0-G1期相关。
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| 关 键 词： | 环氧合酶-2 抑制剂 辐射增敏 细胞周期 P21 胰腺癌细胞 |
Study of enhancement of radiosensitivity in pancreatic carcinoma cell line PC-3 by cyclooxygenase-2 inhibitor |
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| Institution: | (SONG Yipeng,ZANG Rukun,MA Jinbo.Irradiation department of Yantai Yuhuangding hospital,Yantai 264000,China.) |
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| Abstract: | Objective To study the radiosensitizing effect of NS-398,a selective cyclooxygenase-2 inhibitor,on pancreatic carcinoma cell line PC-3 in vitro and investigate possible mechanisms.Methods Pancreatic carcinoma cell line PC-3 were cultured in RPMI1640.flow cytometry analysis(FCM) was used to detect the express of COX-2.MTT assay was performed to evaluate the cytotoxicity of NS-398 and its radiosensitizing effect on PC-3 cell.FCM was applied to explore the change of cell cycle distribution.RT-PCR was applied t... |
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| Keywords: | Cyclooxygenase-2 inhibitors radiosensitivity cell cycle P21 pancreatic cancer |
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