| 建鲤与兴国红鲤RAPD指纹的初步研究 |
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| 引用本文： | 张德春,吕朝阳.建鲤与兴国红鲤RAPD指纹的初步研究[J].南都学坛,2000,20(3):64-65. |
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| 作者姓名： | 张德春 吕朝阳 |
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| 作者单位： | 1. 湖北三峡学院,生物系,湖北,宜昌,443000
2. 河南南阳师专,生物系,河南,南阳,473061 |
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| 摘 要： | 运用 2 0个 10bp随机引物对建鲤 (Cyprinuscarpiovar .jian)和兴国红鲤 (Cyprinuscarpiovar .singuonensis)进行了RAPD -PCR反应。所用引物中 ,除 2个没有扩增产物外 ,其它引物都能对建鲤和兴国红鲤扩增出清晰的RAPD谱带 ,扩增的片段大小在 0 .2kb～ 3.0kb之间。其中 ,8个引物对建鲤个体的扩增有多态现象 ,占引物总数的 40 % ;5个引物对兴国红鲤个体的扩增有多态现象 ,占引物总数的 2 5 %。为建鲤和兴国红鲤RAPD分子标记的建立和品种鉴定提供了基础资料
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| 关 键 词： | 建鲤 兴国红鲤 RAPD |
| 文章编号： | 1002-6320(2000)03-0064-02 |
| 修稿时间： | 1999年5月7日 |
Genetic Analysis of Jian carp and Singuonensis carp by Means of RAPD |
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| Zhang Dechun.Genetic Analysis of Jian carp and Singuonensis carp by Means of RAPD[J].Academic forum of nandu,2000,20(3):64-65. |
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| Authors: | Zhang Dechun |
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| Abstract: | In this study,20 10-mer primers were used for RAPD-PCR reaction in jian carp and singuonensis carp.Only two primers did not produce any band.The other primers produced bands ranging from 0.2 kb to 3.0 kb.Among primer used,8 primers (40% of total 20 primers) produced polymorphic bands among individuals of jian carp,5 primers(25% of total 20 primers) produced polymorphic bands among individuals of singuonensis carp.The study provided basic date for analysis of RAPD marker between jian carp and singwonensis carp. |
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| Keywords: | Jian carp Singwonensis carp RAPD |
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