絮凝天然碱碱泥的生物絮凝剂产生菌分批发酵动力学模型

本论文从生活污水中筛选出一株能产生生物絮凝剂的菌株,经鉴定为肠球菌属(Enterococcus Thiercelin and Jouhaud,1984)中的盲肠肠球菌(E.cecorum),此菌株产生的代谢产物对天然碱碱泥有较好的絮凝效果.利用该菌株进行了生物絮凝剂产生菌分批发酵过程的动力学研究.通过对多次试验数据的拟合,提出了3个细胞生长动力学模型、2个主要基质(麦芽糖)消耗动力学模型、3个产物絮凝剂生成动力学模型,并用统计学软件估算了各动力学模型的模型参数.计算结果表明,各模型的计算值都能较好地与实验结果相吻合,拟合优度R2大于0.9,相对误差基本小于10%.最后,从各类模型中选择了比较有实际意义和应用价值的3个模型分别作为细胞生长、基质消耗和产物生成的动力学模型.

KINETIC MODELS OF BIO-FLOCCULANT-PRODUCING STRAIN UNDER BATCH FERMENTATION

A bio-flocculants-producing strain exhibiting high flocculating activity for clay in natural soda is selected from sewage. The strain is identified as Enterococcus cecorurn. Kinetic studies are made on the bio-flocculants-producing strain under batch fermentation conditions. A series of mathematical kinetic models are built. 3 models are built for describing the process of cell growth. 2 models--for substrate consumption,and 3--for yield of product. New statistic software is employed to determine the parameters of kinetic models. It is proved that the kinetic models show a good agreement between experimental and simulated results. R^2 is larger than 0. 9 and relative error is smaller than 10%. Finally, 3 models with better application value are selected, for cell growth, substrate consumption and yield of product respectively.