| 骨肽对成骨细胞骨形成蛋白2表达影响的实验研究 |
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| 引用本文： | 崔晓明,韩斌.骨肽对成骨细胞骨形成蛋白2表达影响的实验研究[J].西南科技大学学报(哲学社会科学版),2013(5):26-27. |
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| 作者姓名： | 崔晓明 韩斌 |
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| 作者单位： | [1]吉林市人民医院骨二科,130000 [2]吉林市中心医院乳腺甲状腺外科,132001 |
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| 摘 要： | 目的 研究骨肽对成骨细胞骨形成蛋白2（BMP2）表达影响.方法 体外分离培养成骨细胞,0.02mg/ml,0.08mg/ml,0.32mg/ml三个剂量浓度骨肽分别作用24小时,应用流式细胞术检测成骨细胞增殖情况.以上三个剂量浓度骨肽再分别作用成骨细胞24小时、48小时、72小时,收集细胞上清利用酶联免疫法（ELISA法）检测BMP2表达含量.结果 三个尽剂量浓度作用24小时均可引起成骨细胞增殖,并存在剂量依赖性.骨肽各浓度组在24小时、48小时均可引起BMP2表达增高,其中0.08mg/ml剂量组作用48小时效果最为显著.结论 骨肽可促进成骨细胞BMP2蛋白表达,从而发挥促进成骨细胞增殖和分化的作用.
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| 关 键 词： | 骨肽 成骨细胞 骨形成蛋白2（BMP2） |
Experiment study of the effect of peptide on expression of BMP2 in osteoblasts |
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| CUI XiaomingI,Han bin.Experiment study of the effect of peptide on expression of BMP2 in osteoblasts[J].Journal of Southwest University of Science and Technology,2013(5):26-27. |
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| Authors: | CUI XiaomingI Han bin |
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| Institution: | 1. Bone three subject of Jilin People＇s Hospital, Changchun 130000. 2. Breast and thyroid surgery of Jilin Central Hospital, Jilin 132001, China |
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| Abstract: | Objective To research the effect of peptide on expression of BMP2 in osteoblasts. Methods Osteoblasts were cul- tured in vitro. Three dose of peptide were incubated in cell culture media containing 0.02.0.08 and 0.32mg/ml for 24.48 and 72 hours respectively. The expression of BMP2 was determined by Enzyme - linked immunosorbent assays （ELISA）. Results The ex- pression of BMP2 was higher in each dose peptide in 24 and 48 hours. The change of expression of BMP2 was significant in 0. 08mg/ml peptide in 24 hours. Conclusion Peptide can promote proliferation and differentiation of osteoblasts by promoting the expression of BMP2. |
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| Keywords: | Peptide osteoblasts BMP2 |
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