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仙人掌多糖清除活性氧作用研究
引用本文:全光华,兰琦杰,曾富华.仙人掌多糖清除活性氧作用研究[J].湛江师范学院学报,2012,33(3):91-94.
作者姓名:全光华  兰琦杰  曾富华
作者单位:1. 湛江师范学院数学与计算科学学院,广东湛江,524048
2. 湛江师范学院资源植物研究开发中心,广东湛江,524048
摘    要:用超声波浸提醇沉淀法从仙人掌中提取并纯化多糖,采用分光光度法在多种体系中研究了仙人掌多糖清除活性氧的作用,用琼脂糖凝胶电泳法观察了该多糖对·OH导致DNA链损伤的抑制作用.结果表明,仙人掌多糖能够有效地清除O2-和·OH等活性氧,对·OH导致的DNA损伤具有良好的保护效果.

关 键 词:仙人掌多糖  活性氧  抗氧化作用

The Effects of the cactus polysaccharide on Scavenging Reactive Oxygen Species
QUAN Guanghua , LAN Qijie , ZENG Fuhua.The Effects of the cactus polysaccharide on Scavenging Reactive Oxygen Species[J].Journal of Zhanjiang Normal College,2012,33(3):91-94.
Authors:QUAN Guanghua  LAN Qijie  ZENG Fuhua
Institution:1.School of Mathematics and Computational Science,Zhanjiang Normal College, Zhanjiang 524048,Guangdong,China; 2.Plant Engineering Technology Development Center,Zhanjiang Normal College, Zhanjiang 524048,Guangdong,China)
Abstract:Polysaccharide was extracted and purified from cactus.The effects of the cactus polysaccharide on removal of reactive oxygen species in some chemical systems were investigated by spectrophotometry.Inhibitory effect of the cactus polysaccharide on the DNA damage induced by hydroxyl radical was investigated by gel electrophoresis.The result indicated that the cactus polysaccharide could significantly remove O2 and ·OH.This polysaccharide canal so efficiently inhibit DNA damage generated by ·OH.
Keywords:cactus polysaccharide  reactive oxygen species  anti-oxidation
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