| 两种不同淋巴细胞分离液对脐带血单核细胞的提取及后期诱导培养CIK细胞的影响 |
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| 引用本文： | 张校通,赵令卉,王小燕,王翔,申重阳,郑榆坤.两种不同淋巴细胞分离液对脐带血单核细胞的提取及后期诱导培养CIK细胞的影响[J].福建农林大学学报(哲学社会科学版),2011(5). |
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| 作者姓名： | 张校通 赵令卉 王小燕 王翔 申重阳 郑榆坤 |
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| 作者单位： | 成都清科生物科技有限公司; |
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| 摘 要： | 目的:研究两种不同的淋巴细胞分离液对脐带血单核细胞的分离效果及后期对CIK细胞的诱导培养的影响。方法:分别使用国产TBD以及进口GE healthcare牌淋巴细胞分离液分离提取脐带血单核细胞,用悬浮细胞培养法诱导培养脐带血单核细胞形成CIK细胞,再用血球计数板及台盼兰染色法检测细胞密度及活率。结果:国产的TBD牌所分离的单核细胞分层较清楚,并且方便提取,而使用进口GE healthcare的淋巴细胞分离液提取的单核细胞数量较多,但经过后期诱导培养CIK细胞发现两种分离液提取的细胞培养到后期数量逐渐接近。结论:国产TBD牌淋巴细胞分离液在用于脐带血淋巴细胞分离提取时较进口GE healthcare牌经济,并且细胞提取方便,后期培养效果无明显差别。
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| 关 键 词： | 淋巴细胞分离液 脐带血 单核细胞 CIK细胞 |
Effect of Two Different lymphocyte Separatory Liquids on the Extraction of Umbilical Cord Blood mononuclear cells and Later Induction Culture of CIK Cells |
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| ZHANG Xiao-tong,ZHAO Ling-hui,WANG Xiao-yan,WANG Xiang,SHEN Chong-yang,ZHENG Yu-kun.Effect of Two Different lymphocyte Separatory Liquids on the Extraction of Umbilical Cord Blood mononuclear cells and Later Induction Culture of CIK Cells[J].Journal of Fujian Agriculture and Forestry University,2011(5). |
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| Authors: | ZHANG Xiao-tong ZHAO Ling-hui WANG Xiao-yan WANG Xiang SHEN Chong-yang ZHENG Yu-kun |
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| Institution: | ZHANG Xiao-tong,ZHAO Ling-hui,WANG Xiao-yan,WANG Xiang,SHEN Chong-yang,ZHENG Yu-kun(Qing Ke Biological Technology Limited Company,Chengdu 610000,China) |
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| Abstract: | Objective:To compare the result of umbilical cord blood mononuclear cells extracted by two different lymphocyte separation liquids,and the later effect on CIK culture.Methods:The improved methods were used to extract mononuclear cells from umbilical cord blood.Then the mononuclear cells were inducted and cultured to CIK cells.Lastly,the blood corpuscle count plate and trypan blue were used to determination the density and live rate of CIK cells.Results:Compared with the GE healthcare,the quantity of mononuc... |
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| Keywords: | The lymphocyte separation fluid Umbilical cord blood mononuclear cells CIK cells |
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