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| PRRSV JL／07／SW毒株GP5重组蛋白间接ELISA检测方法的建立 | |
| 引用本文： | 高恩鹏,丁壮,高原,孟轲音,宣华,王贵平.PRRSV JL／07／SW毒株GP5重组蛋白间接ELISA检测方法的建立[J].吉林工程技术师范学院学报,2009,25(4):72-74. |
| 作者姓名： | 高恩鹏 丁壮 高原 孟轲音 宣华 王贵平 |
| 作者单位： | 1. 内蒙古民族大学,动物科技学院,内蒙古,通辽,028000 2. 吉林大学,畜牧兽医学院,吉林,长春,130062 3. 军事医学科学院,军事兽医研究所,吉林,长春,130062 4. 广东省农科院兽医研究所,广东,广州,510000 |
| 摘 要： | 本研究将以纯化的重组GP5蛋白作为包被抗原，建立了诊断PRRS的间接ELISA方法。实验确定了最佳抗原包被浓度为2．30μ／mL，PRRSV阳性血清稀释度为1：100。用间接ELISA方法检测猪瘟、猪细小病毒、猪戊肝病毒阳性血清无交叉反应，证明有很好的特异性。 |
| 关 键 词： | 重组质粒 重组GP5蛋白 间接ELISA |
Establishment of GP5-ELISA Diagnosis Method for JL/07/SW Strain of Reproductive and Respiratory Syndrome Virus |
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| GAO En-peng,DING Zhuang,GAO Yuan,MENG Ke-yin,XUAN Hua,WANG Gui-ping.Establishment of GP5-ELISA Diagnosis Method for JL/07/SW Strain of Reproductive and Respiratory Syndrome Virus[J].Journal of Jilin Teachers Institute of Engineering and Technology(Natural Sciences Edition),2009,25(4):72-74. | |
| Authors: | GAO En-peng DING Zhuang GAO Yuan MENG Ke-yin XUAN Hua WANG Gui-ping |
| Institution: | GAO En - peng, DING Zhuang, GAO Yuan , MENG Ke - yin, XUAN Hua, WANG Gui - ping ( 1. College of Animal Science and Technology, Inner Mongolia University for Nationalities, TongLiao 028000, China ; 2. College of Animal Science and Veterinary Medicine, JiILin University, Changchun Jilin 130062,China; 3. Veterinary Institute, Academy of Military Medical Science, Changchun Jilin 130062, China; 4. Cuangdong Veterinary Research Institute, Guangzhou Gnangdong 510000, China ) |
| Abstract: | Using recombined fusion GP5 protein as coating antigen set up a method of indirect enzyme - linked immtmosorbent assay （ELISA） to diagnose PRRSV. The experiment made sure of the optimal primordial covering concentration was 2.30 μg/mL. The optimal dilution of serum examinod was 1： 100. To check out among PPI（poreine parvovims infection） ; CSF（Classical swine fever ）and HEV（hybrid electric vehicle） positive serum, which weren＇t have no cross reaction by indirect ELISA method, it improved that this method had strong specificity. |
| Keywords: | recombinant plasmid recombinant GP5 protein Indirect ELISA |
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