| 硅素在水稻花药培养形成愈伤组织中的作用研究 |
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| 引用本文： | 张跃非,张超英.硅素在水稻花药培养形成愈伤组织中的作用研究[J].西南科技大学学报(哲学社会科学版),1999,16(2):11-13. |
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| 作者姓名： | 张跃非 张超英 |
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| 作者单位： | 绵阳市农业科学研究所!绵阳621000 |
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| 摘 要： | 对 4份采自绵阳市农科所的水稻杂交组合进行花药培养 ,诱导培养基中施加不同硅素浓度处理 ,发现在诱导阶段和分化阶段都明显地提高了诱导频率和分化频率 ,效果以 75mg·l- 1 为好 ,而对绿苗的壮苗阶段影响相对较小 ,ZR973-4 7和W971 -2 1两材料花粉苗每丛苗的绿苗数分别比对照提高 1 6.72 %和8.72 %。结果还表明 ,硅素不能根本改变影响愈伤组织形成和分化的生理生化特性 ,即硅素不是提高难培养材料成苗的限制因子
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| 关 键 词： | 硅素 水稻 花药培养 愈伤组织 培养基 |
The Role of Silicon in Callus formation in Rice Anther Culture |
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| Zhang Yuefei,Zhang Chaoying.The Role of Silicon in Callus formation in Rice Anther Culture[J].Journal of Southwest University of Science and Technology,1999,16(2):11-13. |
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| Authors: | Zhang Yuefei Zhang Chaoying |
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| Abstract: | Applying Silicon with different concentrations in anther culture of 4 hybrid rice crosses could enhance the frequency of induction and differentiation of callus.The best effect occured at the concentration of 75 mg/l,in which the number of green seedling per cluster of anther plants of ZR973-47 and W971-21 was increased by 16.72% and 8.72%,respectively,compared with check.The reserch result,meanwhile, also showed that Silicon could not changed fundamentally physiological and biochemical characteristics of callus formation and differentiation. |
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| Keywords: | Silicon paddy rice anther culture callus culture medium |
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