电针治疗对大鼠脑缺血再灌注中AnnexinAl和TRPM7表达的影响

目的：电针治疗在脑缺血再灌注损伤中AnnexinA1与TRPM7共存为探讨TRPM7在脑缺血再灌注损伤中的途径提供理论依据。方法将SD大鼠随机分成正常对照组、缺血再灌组、电针治疗组、假手术组，以线栓法建立大鼠大脑中动脉栓塞再灌注模型，电针穴位选择“水沟”、“承浆”穴。用Morris水迷宫试验验证学习记忆能力和免疫荧光双标方法检测AnnexinA1和TRPM7在大鼠脑组织中的共存表达。结果①大鼠大脑中动脉栓塞60分钟再灌注24小时后，可出现明显学习记忆能力障碍，缺血再灌组与电针治疗组有显著性差异（P＜0.05），在脑缺血前后以电针治疗后可显著改善大鼠学习记忆能力。②在海马CA1、CA3、皮质区，脑缺血再灌组与正常对照组相比， AnnexinA1和TRPM7共存表达升高率分别是48.06％，50.19％，30.07％（ P＜0.05）。电针治疗组与脑缺血再灌组相比，AnnexinA1和TRPM7共存表达水平下降率分别是34.88％，47.80％，41.96％（ P＜0.05）。结论脑缺血再灌注后大鼠学习记忆能力下降，且AnnexinA1和TRPM7共存表达上调，电针治疗后能够显著改善大鼠的学习记忆能力，并通过逆转AnnexinA1和TRPM7表达，且可能通过TRPM7途径来调整AnnexinA1的作用。电针有可能通过抑制TRPM7和AnnexinA1来改变神经元的凋亡命运。

Effect of electroacupuncture on expression of annexin A1 and TRPM7 after cerebral ischemia/reperfusion in rats

Objective To explore the possible mechanism and pathway of the TRPM 7 in cerebral ischemia/reperfusion injury , the concomitance expression of AnnexinA 1 and TRPM7 provide a theoretical basis.Methods S-D male rat was randomized into normal control group ,ischemia reperfusion group ,electroacupuncture treatment group ,sham operatison group.MCAO model was es-tablished by using the method of thread embolism.The acupucture points of Shuigou and Changjiang were selected.Morris water maze was applied to measure the ability of study and memory.The concomitance expression of AnnexinA 1 and TRPM7 was tested by Im-munofluorescence diplo -scale in the frozen rat brain slice.Results ①MACO for 60min and reperfusion for 24h,all rats appear neurologic defect of the ability of study and memory.The score of electroacupuncture therapy group is lower than that of the ischemia reperfusion group（P〈0.05）.That means electroacupuncture treatment can mitigate the neurologic defect.②In the CA1,CA3 region of hippocampus and the brain cortex the concomitance expression of Annexin A 1 and TRPM7 in the ischemia reperfusion group are higher 48.06%,50.19%,30.07%than that in the normal control group （P〈0.05）.The same concomitance in the electroacupunc-ture therapy group are lower 34.88%,47.80%,41.96%than that in the ischemia reperfusion group （ P〈0.05 ） .Conclusion ①After brain ischemia reperfusion,all rats appear neurologic defect of the ability of study and memory ,electroacupuncture treatment can mitigate the neurologic defect.②After brain ischemia reperfusion , the concomitance expression of AnnexinA 1 and TRPM7 in-creased.Electroacupucture deteriorated the change ,so electroacupuncture may play a role in the stroke through TRPM 7 to regulate the anti-inflammation function of AnnexinA 1.Furthermore electroacupuncture may alter the fate of neuron apoptosis through the reg -ulation of the transloction of TRPM 7 and AnnexinA1 in stoke.