生物安全实验室活毒废水处理工艺研究

目的在借鉴吸收国外生物安全实验室（BSL）活毒废水处理工艺技术的基础上，结合我国国情，研究开发节能、环保、安全型成套处理技术工艺和设备，实现关键技术和产品国产化。方法处理系统采用序批式工艺，通过高温高压方式消毒灭菌。结果该处理工艺的技术指标：灭菌温度100～150℃（可调），加热时间≤30rain，灭菌时间30—120min（可调）；处理后排放指标：活微生物为0，温度≤40℃，pH=6～9。结论该处理工艺整体设计简便灵活，符合BSL设计越简单越安全的理念，适合我国国情，可用于生物安全三级、四级实验室（BSL一3，-4）活毒废水处理。

Treatment of waste water with active virus in bio-safety laboratories

Objective To develop a whole set of energy-conserving, environmentally friendly and safe technology in order to localize key techniques and products by learning from the foreign technologies for handling waste water with active virus in bio-safety laboratories. Methods This treatment system adopted the sequencing batch technology and was sterilized through high temperature and high pressure. Results The technical requirement of treatment was a sterilization temperature of 100 - 150C （ adjustable）, heating time 〈30 min, and sterilization time 30 - 120 min （adjustable）. The effluent index after treatment was： no microbe, temperature 〈40C, and pH = 6 -9. Conclusion The design of the treatment technolo- gy is simple, user-friendly, flexible, and in line with the bio-safety laboratory （BSL） design concept that the simpler, the safer. It also conforms to the situation in China and can be applied to handling waste water with active virus in the level three or four national BSL（BSL-3,-4）.