| D-柠檬烯对人胃癌MGC803细胞增殖和凋亡的影响 |
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| 引用本文： | 王玲,张秀珍.D-柠檬烯对人胃癌MGC803细胞增殖和凋亡的影响[J].福建农林大学学报(哲学社会科学版),2009(1). |
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| 作者姓名： | 王玲 张秀珍 |
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| 作者单位： | 山东理工大学生命科学院; |
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| 基金项目： | 山东理工大学博士启动基金项目(4041-406022) |
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| 摘 要： | 目的研究D-柠檬烯对人胃癌MGC803细胞生长抑制和凋亡的影响。方法噻唑蓝(MTT)法比色检测细胞生长情况;流式细胞仪检测细胞凋亡率;激光共聚焦检测细胞内活性氧(reactive oxygen species,ROS)的含量和caspase- 3蛋白的表达。结果D-柠檬烯对人胃癌MGC803细胞生长抑制作用呈剂量依赖关系;流式细胞仪检测发现D-柠檬烯可引起MGC803细胞凋亡,且呈时间依赖性;激光共聚焦显微镜荧光检测发现D-柠檬烯处理的细胞内ROS明显升高(P<0.05),caspase-3蛋白表达明显增加(P<0.05)。结论D-柠檬烯能够有效地抑制癌细胞的生长以及诱导凋亡。这种作用机制可能是使MGC803细胞内产生大量的活性氧。也可能与细胞内caspase-3基因的表达变化有关。
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| 关 键 词： | D-柠檬烯 人胃癌MGC803细胞 凋亡 |
Growth inhibition effects of D-limonene on Human Gastric Carcinoma MGC803 cells |
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| Wang Ling Zhang Xiu-zhen.Growth inhibition effects of D-limonene on Human Gastric Carcinoma MGC803 cells[J].Journal of Fujian Agriculture and Forestry University,2009(1). |
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| Authors: | Wang Ling Zhang Xiu-zhen |
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| Institution: | Department of Life Science;Shandong University of Technology;zibo 255049;P.R.China |
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| Abstract: | Objective To investigate the antieancer effect of D-limonene on Human Gastric Carcinoma MGC803 cells.Methods Cell growth inhibition was measured by MTT colorimetric assay;apoptosis rate was measured by flowcytometry(FCM);laser scanning confocal microscopy (LSCM)was used to detect the reactive oxygen species(ROS)and the expression status of caspase-3 proteins.Results Growth inhibition of D- limonene on MGC803 cells was in a dose dependent manner;FCM detected that d-limonene have induced apoptosis in a time d... |
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| Keywords: | D-limonene Human Gastric Carcinoma MGC803 cells apoptosis |
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