| 毛叶秋海棠离体繁殖技术研究 |
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| 引用本文： | 孔祥生,张妙霞,刘宗才. 毛叶秋海棠离体繁殖技术研究[J]. 南都学坛, 1999, 19(6): 53-55 |
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| 作者姓名： | 孔祥生 张妙霞 刘宗才 |
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| 作者单位： | 1. 洛阳农业高等专科学校,洛阳,471003
2. 南阳师专生物系,南阳,473061 |
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| 摘 要： | 以毛叶秋海棠的幼叶为外植体接种在附加BA0．5－2．5mg·L-1和NAA0．1mg·L-1的MS培养基上，能诱导产生大量不定芽。采用MS＋BA0．5mp·L-1＋NAA0．1mg·L-1的培养基进行继代培养，每4－6周可扩大增殖10倍左右。将幼芽转入无生长调节剂的MS培养基中，6d即可生根。生根的毛叶秋海棠试管苗移栽成活率可达90％。
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| 关 键 词： | 毛叶秋海棠 芽形成 离体繁殖 |
| 修稿时间： | 1998-12-09 |
In Vitro Propagation of Begonia Rex |
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| Kong Xiangsheng,Zhang Miaoxia. In Vitro Propagation of Begonia Rex[J]. Academic forum of nandu, 1999, 19(6): 53-55 |
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| Authors: | Kong Xiangsheng Zhang Miaoxia |
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| Abstract: | This paper reports the method for in vitro propagation of in vitro propagation of Begonia rex. The explants taken bo young leaves of Begonia rex produced a lot of adventitious buds on MS medium supplemented with 0.5 - 2.5 mg·L-1 BA and 0. 1 mg·L-1 NAA. MS + BA0.5 mg.L-1 + NAA. 1 mg.L-1 medium was used in continuous culture, buds with in the medium multiplied 10 times or so per 4 - 6 weeks. It rooted in 6 days after buds were transffered on growth-regulators-free MS medium. Survival rate of plantlets which have been trans-planted in the humus soil and vermiculite could reach 90%. |
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| Keywords: | Begonia rex Buds formation In vitro propagation |
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