| HPLC法测定小儿消食片中橙皮苷的含量 |
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| 引用本文： | 陈明,龙子江.HPLC法测定小儿消食片中橙皮苷的含量[J].福建农林大学学报(哲学社会科学版),2006(5). |
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| 作者姓名： | 陈明 龙子江 |
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| 作者单位： | 安徽中医学院中西医临床结合学院 安徽合肥230038 |
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| 摘 要： | 目的采用HPLC法测定小儿消食片中橙皮苷的含量。方法HPLC法,VP-ODS柱(4.6mm*250mm),流动相:乙腈-0.4%磷酸溶液(24:76),检测波长:283nm,流速:1.0ml·min-1;结果橙皮苷在1.56μg～15.61μg范围内有良好的线性关系,r=0.9999(n=5),平均回收率为98.79%。结论该方法分离度高,重现性好,简便,准确,可用于小儿消食片的质量控制。
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| 关 键 词： | HPLC 小儿消食片 橙皮苷 |
Determination of Hesperidin in Xiaoerxiaoshi Tablets by HPLC |
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| Ming Chen,Zijiang Long.Determination of Hesperidin in Xiaoerxiaoshi Tablets by HPLC[J].Journal of Fujian Agriculture and Forestry University,2006(5). |
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| Authors: | Ming Chen Zijiang Long |
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| Abstract: | OBJECTIVE To determine hesperidin in Xiaoerxiaoshi tablets by HPLC. METHODS HPLC was used in the quantitative analysis of hesperidin in the sample.The separation was performed on VP-ODS C18(4.6*250mm,5um)column with a mixture of acetonitrile-0.4%phosphoric acid(24:76) as mobile phase.The detection wavelengh was set at 283 nm and the flow rate was 1.0ml.min-1. RESULTS The standard curve was linear in the range of 1.56-15.61ug for hesperidin (r=0.9999,n=5).The average recovery was 98.79%. CONCLUSIONS The method is simple,reliable,and is a good method to determinate hesperidin. |
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| Keywords: | HPLC Xiaoerxiaoshi tablets Hesperidin |
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