南蛇藤果中内生真菌的抑菌活性研究

对从南蛇藤果实中分离的16株内生真菌用马铃薯葡萄糖培养基进行液体培养,将发酵产物中的发酵液用高压蒸汽湿热灭菌,菌丝体晾干研磨后用丙酮提取.用发酵处理液和丙酮粗提物对一些常见的植物病原菌进行抑菌活性测定.结果表明,发酵产物的抑菌率在50%以上的活性菌株有13株(占81.2%).说明南蛇藤内生真菌中的抗病原真菌资源十分丰富,其中抑菌活性最强的发酵处理液的抑菌率高达90.5%,菌丝粗提物的抑菌率达89.2%,但活性菌株的抗菌谱比较狭窄.

Study on the Antifungal Activities of Endophytic Fungi Isolated from the fruits of Celastrus orbiculatus Thunb

Sixteen isolates of endophytic fungi were obtained from the fruits of Celastrus orbiculatus Thunb,which were cultivated in liquid media respectively.Moist-heat sterilization was used to dispose the fermentation broth,and mycelia were extracted with acetone after desiccated and grounded.The antifungal activities of the disposed fermentation broth and the acetone extracts on several plant pathogenic fungi were measured.The results show that the fermentation products of 13 strains(81.2%)exhibits more than 50% fungicidial activity.The endophytic fungi with antifungal activities in C.orbiculatus are resourceful.The inhibition rate of the disposed cultural liquid with the strongest antifungal activity is as high as 90.5%,while that of the mycelium extract is up to 89.2% and the antifungal spectrum was narrow.