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| 凋亡通路蛋白表达对过氧化氢诱导PC12细胞凋亡的影响 | |
| 引用本文： | 薛宏宇,李晓宇,李卫东,李霞荣,陈红玲. 凋亡通路蛋白表达对过氧化氢诱导PC12细胞凋亡的影响[J]. 宿州学院学报, 2011, 26(2): 40-43,107. DOI: 10.3969/j.issn.1673-2006.2011.02.015 |
| 作者姓名： | 薛宏宇 李晓宇 李卫东 李霞荣 陈红玲 |
| 作者单位： | 1. 宿州学院化学与生命科学学院,安徽宿州,234000;大连理工大学环境与生命学院生物科学与工程系,辽宁大连,116024 2. 大连理工大学环境与生命学院生物科学与工程系,辽宁大连,116024 3. 宿州学院化学与生命科学学院,安徽宿州,234000 |
| 基金项目： | 宿州学院教授(博士)启动基金，安徽省自然科学基金资助项目，安徽省高等学校优秀青年人才基金，安徽省高等学校省级自然科学研究项目 |
| 摘 要： | 目的探讨过氧化氢（H2O2）诱导PC12细胞凋亡的机制。方法 MTT测定细胞存活率,荧光染色观察细胞形态,透射电镜观察细胞的超微结构,流式细胞仪测定细胞凋亡率,Western blotting技术和流式细胞仪检测Bcl-2和Bax蛋白的表达,分光光度计测定Caspase-3释放率。结果 0.25 mM的H2O2可诱导PC12细胞凋亡,细胞的形态发生改变,细胞核受损,细胞内Bcl-2蛋白的表达降低、Bax蛋白的表达增加,Caspase-3被激活。结论 H2O2诱导了PC12细胞凋亡,其作用机制与细胞内Bcl-2、Bax蛋白的表达及Caspase-3活性密切相关。 |
| 关 键 词： | 过氧化氢 PC12细胞 凋亡 |
Expression of Ap0ptosis Pathway Proteins Modulates H2O2-induced Apopt0sis in PC12 Cells |
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| XUE Hong-yu,LI Xiao-yu,LI Wei-dong,LI Xia-rong,CHEN Hong-ling. Expression of Ap0ptosis Pathway Proteins Modulates H2O2-induced Apopt0sis in PC12 Cells[J]. Journal of Shuzhou College, 2011, 26(2): 40-43,107. DOI: 10.3969/j.issn.1673-2006.2011.02.015 | |
| Authors: | XUE Hong-yu LI Xiao-yu LI Wei-dong LI Xia-rong CHEN Hong-ling |
| Affiliation: | 1.Department of Chemistry and Life Science,Suzhou University,Suzhou,Anhui 234000;2.Department of Bioscience and Biotechnology,School of Environmental and Biological Scienceand Technology,Dalian University of Technology,Dalian,Liaoning,116024,China |
| Abstract: | Objective:To investigate the apoptotic effect of H2O2 in rat pheochromocytoma(PC12) cell line.Methods The cell viability was analyzed by MTT;the morphological changes were observed by Hoechst 33 258 staining analysis;the ultrathin sections of cells were observed by transmission electron microscopic(TEM);the apoptosis rate,the protein of Bcl-2 and Bax were measured by the flow cytometry analysis(FCM and Western blotting);the Caspase-3 activation was measuredusing Microplate Fluorometer.Results:H2O2(0.25 mM) could result in the decrease of cell viability,the increase of Bax and the dropping of Bcl-2 protein expression of PC12 cell.It increased the activity of Caspase-3 and the apoptotic rate of cells.Conclusions:The apoptosis in H2O2-induced PC12 cells was accompanied by down-regulation of Bcl-2,up-regulation of Bax and sequential activation of Caspase-3. |
| Keywords: | Hydrogen Peroxide PC12 Cell Apoptosis |
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