| 苜蓿花叶病毒RNA_23′端cDNA转基因烟草植株的抗病性分析 |
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| 引用本文： | 陈秀莉.苜蓿花叶病毒RNA_23′端cDNA转基因烟草植株的抗病性分析[J].阴山学刊,1999(6). |
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| 作者姓名： | 陈秀莉 |
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| 作者单位： | 包头师范高等专科学校生物学系 内蒙古 |
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| 摘 要： | 将人工合成的与苜蓿花叶病毒(ALMV)RNA_2互补的寡核苷酸进行放射性同位素(γ-P~(32)ATP)标记,制备成探针。用ALMV攻毒转基因烟草和未转基因烟草,提取总RNA,用上述探针分析植珠体内病毒增殖情况,做抗病性分析。实验表明:转基因植珠(李颖同学做的转基因植株)有较强的抗病性,而未转基因的对照株却无抗病性。
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| 关 键 词： | 苜蓿花叶病毒 RNA_2 转基因植珠 |
Alfalfa Mosaic Virus RNA_23'cDNA Trancplated Gene Cigarette's Disease Resistance Analysing |
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| Chen Siuli.Alfalfa Mosaic Virus RNA_23'cDNA Trancplated Gene Cigarette's Disease Resistance Analysing[J].Yin Shan Academic Journal,1999(6). |
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| Authors: | Chen Siuli |
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| Abstract: | After being signed with radioactive elements(r-32 ATP)matched with Al-falfa mosaic virus ribonucleic acid-2 can be made into probe. Extract the general ri-bonucleic acid from the transplanted gene cigarette which is infected with Alfalfa mosaic virus and the untransplanted gene cigarrette. Analyse the virus multiplication within the plant and disease resistance with that probe. It is proved that transplanted gene plant has strong disease resistance ability while the untransplanted plant has none. |
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| Keywords: | alfalfa mosaic virus jribonucleic acid transplanted gene cigarette |
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