| 水解叶蛋白的高效蛋白酶的筛选研究 |
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| 引用本文： | 杨卫韵.水解叶蛋白的高效蛋白酶的筛选研究[J].绍兴文理学院学报,2004,24(8):44-47. |
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| 作者姓名： | 杨卫韵 |
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| 作者单位： | 金华职业技术学院,生物工程学院,浙江,金华321007 |
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| 摘 要： | 选择3种不同来源的蛋白酶，即碱性蛋白酶、胰蛋白酶、木瓜蛋白酶，利用正交试验L9(3^4)考察它们对叶蛋白水解的催化效能的高低及反应时间、温度及pH三因素对酶促水解的影响作用．结果表明，3种酶中碱性蛋白酶的效能最高；反应时间、温度及pH三因素中，影响最大的是pH值；叶蛋白适于偏碱性条件下水解．
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| 关 键 词： | 水解 叶蛋白 高效蛋白酶 筛选 |
| 文章编号： | 1008-293X(2004)08-0044-04 |
| 修稿时间： | 2004年3月20日 |
Study on Selecting the Effective Protease for Leaf Protein's Hydrolysis |
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| Yang Weiyun.Study on Selecting the Effective Protease for Leaf Protein''''s Hydrolysis[J].Journal of Shaoxing College of Arts and Sciences,2004,24(8):44-47. |
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| Authors: | Yang Weiyun |
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| Abstract: | The leaf protein's hydrolysis is catalyzed by three kinds of proteases with different resources,e.g. alkaline protease,trypsin and papain,and their catalytic activity are studied in the orthogonal test (L 9(3 4));Effects of infl uencing factors on hydrolysis,including pH value,temperature,and hydrolysis t ime,are also investigated. The result reveals: the alkaline protease is the one with the highest activity among them;within the three factors,pH value is the most effective;and the optimal pH value of the leaf protein's hydrolysis is un der alkaline condition. |
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| Keywords: | hydrolysis of leaf protein protease selection |
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