猪水肿毒素琼脂扩散沉淀反应方法的建立

采用滤膜除菌、生物大分子浓缩胶浓缩、戊二醛灭活的方法制备的EHEc水肿毒素抗原,可与其兔高免血清和鸡高免血清发生沉淀反应。对5株EHEc进行了分析,并与小白鼠毒性试验结果进行了比较,证明该沉淀反应具有高度的特异性。     

应用凝胶电泳分析法对平菇亲缘关系早期鉴定的研究

应用改进的聚丙烯酰胺凝胶电泳分离技术,对不同平菇品种菌丝体过氧化物酶同工酶和可溶性蛋白质谱带的测定结果表明:供试的各品种之间在同工酶和蛋白质谱带的数目、相对迁移率(Rf 值)、酶活性以及蛋白质量等方面均存在着显著的差异,因此可以作为平菇属种间亲缘关系早期鉴定的生化指标。与传统的菇形,色泽等形态学特征辨认及子实体等组织电泳分析相比较,本方法更具有简便、快速、可靠、灵敏等优点。对于伪劣、混杂、退化及变异菌株的早期检测,在食用菌的生产、销售和育种上都具有重要的应用价值。     

Optimal lamination test of ethylene vinyl acetate sheets for solar modules

ABSTRACT

Solar power is inexhaustible and has become one of the most appreciated alternative energy choices. In the development stage, solar modules are subjected to relevant reliability tests to ensure a long lifetime and optimal power generation efficiency. After the lamination process, the performance of solar modules is closely related to the degree of crosslinking of ethylene vinyl acetate (EVA) sheets. Traditionally, the degree of crosslinking on EVA sheets is obtained using the chemical extraction method to measure the gel content of these sheets. Motivated by lamination data, this study first constructed a statistical model to describe the relationship between the degree of crosslinking on EVA sheets and lamination time. Next, under the specification limits of the gel content of EVA sheets, the optimal lamination time of solar modules was derived, and the optimal allocation for measuring EVA sheets was addressed. The chemical extraction method is time consuming and leads to high pollution. The latest method is differential scanning calorimetric (DSC), which measures the curing degree of EVA sheets as the degree of crosslinking on these sheets. This study determined the specification limits of the curing degree under the DSC method. An example is presented to elucidate the proposed procedure.     

交联聚合物动态成胶及运移的室内模拟研究

室内采用长岩芯填砂装置模拟了交联聚合物注入、成胶封堵和运移过程。室内模拟结果表明,注入的调剖体系能够达到设计的封堵位置,在注入过程中体系受稀释、剪切和吸附等因素影响,其浓度和成胶前的初始黏度沿程呈下降趋势。在实验所用的浓度（0.2%HPAM）条件下,孔隙介质中的堵剂体系形成有效堵塞的时间显著延长,是静态条件下的6 倍;从不同部位堵剂取样成胶后黏度和岩芯封堵前后注水压力变化情况看,有效封堵距离r 小于设计封堵距离r′,0.67r′ < r < r′。在后续水驱作用下,一部分凝胶能够沿高渗层发生运移,产生深部调驱作用。     

Effects of testosterone treatment on body composition in males with testosterone deficiency syndrome

Abstract

Objective: We evaluated the safety of testosterone treatment and its efficacy on body composition in males with testosterone deficiency syndrome (TDS) over 24 months.

Methods: 50 males aged 50–65 years with TDS (Aging Males Symptoms Scale [AMS]?>?26 and calculated free testosterone [cFT] 250?pmol/l) were administered 50?mg testosterone gel daily for one year. During the second year, patients received 1000?mg of testosterone undecanoate every 2–3 months. Outcome measures were clinical chemistry values and total testosterone; sex hormone-binding globulin and cFT, changes in AMS and International Prostate Symptom Score; and changes in body composition measured by dual-energy-x-ray absorptiometry.

Results: There were no clinically significant changes in clinical chemistry safety parameters. There were significant improvements in both total and cFT and in AMS scores after three months (p?<?0.001). Lean mass increased 2.35% at 12 months and 4.5% at 24 months, but proportionally more muscle mass was gained in arms and legs than in the trunk. Fat mass decreased 4.2% at 12 months and 9.1% at 24 months.

Conclusions: Testosterone treatment in males with TDS leads to body changes affecting lean and fat mass with significant improvement in AMS scores, and has an excellent safety profile.     

毛细管区带电泳法间接测定红细胞中的无机阳离子

建立了毛细管区带电泳法间接测定七种无机阳离子的方法,考察了背景溶液pH值、咪唑和酒石酸浓度等对无机离子分离效果及峰面积的影响。以10mmol/L咪唑-4mmol/L酒石酸(pH=5.5)为背景,在15kV、214nm的条件下,对红细胞消化液中K+、Mg2+、Ca2+和Zn2+进行了定性、定量分析。回收率在96%～104%之间,线性相关系数均在0.9990以上,迁移时间和峰面积的日内相对标准偏差分别低于0.76%和2.83%,日间相对标准偏差分别低于0.85%和3.69%。本方法弥补了原子吸收法不能同时测定细胞内多种离子的不足,有望成为测定细胞内离子含量的检测方法之一。     

我国毛细管电泳文献分析及趋势预测(1989-1996)

本文用文献计量学的统计方法对《中文科技期刊数据库》1989 - 1996 年间收录我国毛细管电泳的专题论文进行了统计分析,旨在揭示我国毛细管电泳研究的现状及发展趋势,为情报服务及科研选题提供科学依据.     

毛细管电泳单细胞分析方法及应用进展

本文对毛细管电泳用于单个细胞分析的方法及应用进行综述。较系统地论述了毛细管电泳分析时单个细胞取样、溶膜、衍生和检测的方法,以及应用新进展。     

小鹅瘟病毒的增殖与沉淀抗原的制备

取小鹅瘟病毒经尿囊腔接种12日龄非免疫鸭胚进行传代增殖，收集尿囊液后采用聚乙二醇沉淀法制备小鹅瘟病毒沉淀抗原，经琼脂扩散试验检测，结果表明：该抗原能够与小鹅瘟阳性血清出现沉淀线，而且与小鹅瘟标准抗原的沉淀线相吻合，与其他常见的禽病血清不发生交叉沉淀反应．本次制备的小鹅瘟沉淀抗原可以用于免疫效果的监测和流行病学调查。     

产卵期东方蝾螈(Cynops orientalis)同工酶的分析研究

采用聚丙烯酰胺垂直平板电泳方法，研究了东方蝾螈七种组织（小肠、肝、肾、脑、心脏、性腺和尾肌）中的LDH同工酶和EST同工酶，电泳结果显示，不同个体不同组织的LDH同工酶均有二条酶带（LDH1和LDH5），数量上组织特异性不明显，但在显色深度上存在组织差异，其中性腺的LDH1带显色最浅，这表明组织细胞中同时合成了A和B亚单位，但并非随机组合为五种四聚体，显示出经典的五条带．EST同工酶谱可分为四个区共19条带，具有明显的组织特异性，雌雄个体同组织间的差异也很显著。